Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods

<p>1. How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp<br>J. Sung, K.I. Mortensen, J.A. Spudich and H. Flyvbjerg<br>2. Studying the Mechanochemistry of Processive Cytoskeletal Motors with an Optical Trap<br>V. Belyy and A. Yildiz<br>3. Single-Molecule Optical Trapping Techniques to Study Molecular Mechanisms of a Replisome<br>B. Sun and M.D. Wang<br>4. Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy<br>M. H. Shabestari, A.E.C. Meijering, W.H. Roos, G.J. L. Wuite and E.J.G. Peterman<br>5. Direct Visualization of Helicase Dynamics Using Fluorescence Localization and Optical Trapping<br>C.-T. Lin and T. Ha<br>6. High-Resolution Optical Tweezers Combined with Single-Molecule Confocal Microscopy<br>K.D. Whitley, M.J. Comstock and Y.R. Chemla#<br>7. Integrating Optical Tweezers, DNA Tightropes and Single Molecule Fluorescence Imaging: Pitfalls and Traps<br>J. Wang, J.T. Barnett1, M.R. Pollard and N.M. Kad<br>8. Single-Stranded DNA Curtains for Studying Homologous Recombination<br>C.J. Ma, J.B. Steinfeld and E.C. Greene<br>9. Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of  DNA Mismatch Repair<br>M.W. Brown, A.D.L. Torre and I.J. Finkelstein<br>10. Single-Molecule Insight into Target Recognition by CRISPR-Cas Complexes<br>M. Rutkauskas, A. Krivoy, M. Szczelkun, C. Rouillon and R. Seidel<br>11. Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules<br>C. Duboc, J. Fan, E.T. Graves and T.R. Strick<br>12. Measuring Force Induced Dissociation Kinetics of Protein Complexes Using Single Molecule Atomic Force Microscopy<br>K. Manibog, C.-F. Yen and S. Sivasankar<br>13. Improved Force Spectroscopy Using Focused-Ion-Beam Modified Cantilevers<br>J.K. Faulk, D.T. Edwards, M.S. Bull and T.T. Perkins<br>14. Single-Molecule Characterization of DNA-Protein Interactions Using Nanopore Biosensors<br>A.H. Squires, T. Gilboa, C. Torfstein, N. Varongchayakul and A. Meller<br>15. Subangstrom Measurements of Enzyme Function using a Biological Nanopore, SPRNT<br>A.H. Laszlo, I.M. Derrrington and J.H. Gundlach<br>16. Multiplexed, Tethered Particle Microscopy for Studies of  DNA-Enzyme Dynamics <br>S. Ucuncuoglu, D.A. Schneider, E.R. Weeks, D. Dunlap and L. Finzi</p>